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Coreceptor usage and sensitivity to neutralization of HIV-1 and HIV-2
(2004-11-05)
The aim of this project was to study neutralization and coreceptor use in HIV-1 and HIV-2. The first step was to develop a simple and standardized assay for detection and quantification of HIV- 1 neutralizing activity in human sera. The assay...
mPGES-1 and the PGE2 pathway in arthritis
(2005-11-24)
or pathological stimulation. Microsomal prostaglandin E synthase 1 (mPGES-1) constitutes an enzyme responsible for the inducible production of prostaglandin E2 (PGE2), a prostaglandin thought to play an important role in the pathogenesis of arthritis.
In our...
Metalloproteinases in development and disease
(2004-08-06)
. Membrane-type 1 matrix metalloproteinase (MT1-MMP), encoded by MMP14, was firstly identified in invasive tumor cells to serve as the receptor and activator of proMMP2.
To investigate the biological functions of MT1-MMP, we generated Mmp14 knock-out mice...
Wnt signalling in the development of ventral midbrain dopaminergic neurons
(2004-12-24)
in the developing rat VM, with a sequential expression of Dkk-1, Dkk-2 and LRP-6 (both peaking at the time of DA neurogenesis) and Dkk-3. Dkks distinctively regulated DA differentiation from E14.5 VM precursors.
Dkk-1, a Wnt/beta-catenin signalling inhibitor...
Molecular aspects of proinsulin C-peptide interactions
(2006-05-10)
-signal regulated kinase (ERK) 1/2 correlates with the presence of residues Glu-3, -11 and -27 as well as non-helix-breaking residues in the N-terminal third of C-peptide, which forms a helical structure in trifluoroethanol. A tripartite model of C-peptide activity...
Native protein mass spectrometry of nuclear receptor-ligand and enzyme-substrate complexes
(2004-11-05)
in this thesis focuses on the use of mass spectrometry as a method to detect binding of low molecular weight compounds to proteins such as nuclear receptors and enzymes.
The retinoid X receptor alpha (RXRalpha, NR2B1) is a receptor for 9-cis-retinoic acid...
Leukotriene A4 hydrolase : studies of structure-function relationships by site-directed mutagenesis and X-ray crystallography
(2004-10-15)
. Furthermore, protein-ligand
interactions are confirmed by the LTA4H-HACAP crystal structure complex
at 2.0 A resolution....
Diet and renal cell carcinoma
(2005-01-14)
Renal cell carcinoma (RCC) represents 80-85% of all tumors of the kidney and is responsible for about 2% of all cancer deaths in developed countries. Its incidence varies among countries, among migrants, and by socioeconomic status suggesting...
Synthesis of indole and oxindole derivates incorporating pyrrolidino, pyrrolo or imidazolo moieties
(2004-11-26)
The focus of this thesis is on the synthesis of oxindole- and indole-derivatives incorporating pyrrolidins, pyrroles or imidazoles moieties.
Pyrrolidino-2-spiro-3'-oxindole derivatives have been prepared in high ...
Protein mass spectrometry in the drug discovery process
(2005-04-22)
Most targets for drug discovery are proteins. Drug discovery includes target identification and validation, structural biology, lead generation, lead optimisation, pre-clinical development and clinical trials. The overall ...