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Native protein mass spectrometry of nuclear receptor-ligand and enzyme-substrate complexes
(2004-11-05)
in this thesis focuses on the use of mass spectrometry as a method to detect binding of low molecular weight compounds to proteins such as nuclear receptors and enzymes.
The retinoid X receptor alpha (RXRalpha, NR2B1) is a receptor for 9-cis-retinoic acid...
Leukotriene A4 hydrolase : studies of structure-function relationships by site-directed mutagenesis and X-ray crystallography
(2004-10-15)
determinants in LTA4H were largely unknown. In
this thesis we present studies of structure-function relationships in
LTA4H using site-directed mutagenesis and X-ray crystallography.
A conserved GXMEN motif was identified in sequence alignments of members...
Expression of the glucose-6-phosphatase system in endocrine cells
(2006-05-26)
Glucose-6-phosphatase (G6pase) is a multi-component enzyme system, which regulates the catalysis of gluco se-6 -phosphate (G6P) to glucose and inorganic phosphate. This reaction constitutes the final steps of both ...
Metalloproteinases in development and disease
(2004-08-06)
Metalloproteinases are Zn-dependent proteases composed of five superfamilies. One important subfamily is the matrix metalloproteinases (MMPs) that process various components of the extracellular matrix (ECM) and non-ECM ...
The post-polio syndrome : studies of immunology and immunomodulatory intervention
(2005-09-02)
Late effects in patients with prior poliomyelitis were first reported in the nineteenth century and have been referred to as the post-polio syndrome (PPS). PPS is characterized by new muscular deterioration, fatigue and ...
Antimicrobial peptides and proteins in innate immunity : emphasis on isolation, characterization and gene regulation
(2005-03-18)
Antimicrobial peptides are endogenous antibiotics and effector molecules
of Innate immunity. These peptides are mainly localised to epithelial
linings and circulating neutrophils. They kill microbes by disruption of
their ...
C-peptide structural and functional relationships studied by biosensor technology and mass spectrometry
(2005-05-26)
Proinsulin C-peptide has a number of biological activities and receives interest focusing on the therapeutic potential as a candidate for future co-replacement therapy with insulin in type 1 diabetes. Based on conservation ...
Protein mass spectrometry in the drug discovery process
(2005-04-22)
Most targets for drug discovery are proteins. Drug discovery includes target identification and validation, structural biology, lead generation, lead optimisation, pre-clinical development and clinical trials. The overall ...
Formyl-coenzyme : a transferase, structure and enzymatic mechanism
(2004-08-20)
Background: Formyl-CoA transferase (Frc) is the first enzyme in a two
enzyme pathway responsible for oxalate degradation in Oxalobacter
formigenes. This bacterium is a constitutive part of human intestinal
flora. Its ...
Processing, stability and interactions of lung surfactant protein C
(2005-11-23)
Mature SP-C is a 4.2 kDa transmembrane protein which is uniquely expressed in the alveolar type II cell. Human SP-C is generated via multistep proteolytic cleavage of both the C-terminal and Nterminal regions of proSP-C. ...